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dc.contributor.authorAnayol, Emine
dc.contributor.authorBakhsh, Allah
dc.contributor.authorKarakoc, Omer Cem
dc.contributor.authorOnarici, Selma
dc.contributor.authorKom, Deniz
dc.contributor.authorAasim, Muhammad
dc.contributor.authorOzcan, Sebahattin
dc.date.accessioned2019-08-01T13:38:39Z
dc.date.available2019-08-01T13:38:39Z
dc.date.issued2016
dc.identifier.issn1863-5466
dc.identifier.issn1863-5474
dc.identifier.urihttps://dx.doi.org/10.1007/s11816-016-0388-5
dc.identifier.urihttps://hdl.handle.net/11480/3684
dc.descriptionWOS: 000373886900005en_US
dc.description.abstractMost of the commercialized Bt crops express cry genes under 35S promoter that induces strong gene expression in all plant parts. However, targeted foreign gene expression in plants is esteemed more important as public may be likely to accept 'less intrusive' expression of transgene. We developed plant expression constructs harboring cry1Ac gene under control of wound-inducible promoter (AoPR1) to confine Bt gene expression in insect wounding parts of the plants in comparison with cry1Ac gene under the control of 35S promoter. The constructs were used to transform four Turkish cotton cultivars (GSN-12, STN-468, Ozbek-100 and Ayhan-107) through Agrobacterium tumefaciens strains GV2260 containing binary vectors p35SAcBAR.101 and AoPR1AcBAR.101 harboring cry1Ac gene under control of 35S and AoPR1, respectively. Phosphinothricin (PPT) was used at concentration of 5 mg L-1 for selection of primary transformants. The primary transformants were analyzed for transgene presence and expression standard molecular techniques. The transformants exhibited appreciable mortality rates against larvae of Spodoptera exigua and S. littoralis. It was found that mechanical wounding of T (1) transgenic plants was effective in inducing expression of cry1Ac protein as accumulated levels of cry1Ac protein increased during post-wounding period. We conclude that use of wound-inducible promoter to drive insecticidal gene(s) can be regarded as a valuable insect-resistant management strategy since the promoter activity is limited to insect biting sites of plant. There is no Bt toxin accumulation in unwounded plant organs, seed and crop residues, cotton products and by-products, thus minimizing food and environmental concerns.en_US
dc.description.sponsorshipScientific and Technological Research Council of Turkey TUBITAK [111O254]en_US
dc.description.sponsorshipThe work on development of transgenic cotton in our laboratory is being supported by grants from Scientific and Technological Research Council of Turkey TUBITAK (Project No. 111O254). The authors acknowledge contribution and support of TUBITAK. The authors are also thankful to Leicester University (UK) for giving permission to use AoPR1 promoter for research purposes.en_US
dc.language.isoengen_US
dc.publisherSPRINGERen_US
dc.rightsinfo:eu-repo/semantics/closedAccessen_US
dc.subjectGenetic modificationen_US
dc.subjectInsect resistanceen_US
dc.subjectConfined expressionen_US
dc.subjectInsect managementen_US
dc.titleTowards better insect management strategy: restriction of insecticidal gene expression to biting sites in transgenic cottonen_US
dc.typearticleen_US
dc.authorid0000-0001-5110-6014en_US
dc.authorid0000-0003-3561-7863en_US
dc.authorid0000-0002-3091-2639en_US
dc.authorid0000-0002-8524-9029en_US
dc.authorid0000-0002-1697-9327en_US
dc.authorid0000-0002-1697-9327en_US
dc.relation.journalPLANT BIOTECHNOLOGY REPORTSen_US
dc.departmentNiğde ÖHÜen_US
dc.identifier.volume10en_US
dc.identifier.issue2en_US
dc.identifier.startpage83en_US
dc.identifier.endpage94en_US
dc.relation.publicationcategoryMakale - Uluslararası Hakemli Dergi - Kurum Öğretim Elemanıen_US
dc.contributor.institutionauthor[0-Belirlenecek]
dc.identifier.doi10.1007/s11816-016-0388-5


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